CD20 Monoclonal Antibody

Biomaterial Description

The CD20 monoclonal antibody (clone 2H7) specifically recognizes the CD20 antigen, a 33–37 kDa non-glycosylated phosphoprotein expressed on human B lymphocytes. CD20 plays a critical role in regulating B cell activation, proliferation, and calcium signaling, and its expression is restricted to the B cell lineage. Clone 2H7 binds to a defined epitope within the large extracellular loop of CD20, ensuring selective recognition of intact B cells in both research and clinical assays. This precise binding profile enables reliable identification, isolation, and functional analysis of B cell subsets, making clone 2H7 a foundational tool for immunophenotyping, diagnostics, and therapeutic development.

Applications

-Immunophenotyping: Distinguish B cells from other lymphoid populations in flow cytometry, immunohistochemistry, and immunofluorescence assays

-B cell biology research: Investigate differentiation pathways, activation states, and blast transformation in human B cells

-Clinical diagnostics: Support classification of B cell malignancies such as non-Hodgkin lymphoma and chronic lymphocytic leukemia

-Therapeutic development: Serve as a research tool in the design and validation of CD20-targeted therapies, including monoclonal antibody drugs (e.g., rituximab analogs)

-Functional assays: Enable depletion or modulation of CD20+ B cells in experimental systems

Advantages

-High specificity: Clone 2H7 binds selectively to CD20 without cross-reactivity to other leukocyte antigens

-Versatility: Compatible with multiple assay formats (flow cytometry, immunohistochemistry, immunofluorescence)

-Research-to-clinic bridge: Provides a foundational reagent for both basic immunology studies and translational oncology applications

-Historical validation: Originates from landmark monoclonal antibody work defining B cell surface molecules, ensuring robust characterization

-Scalable utility: Applicable across diverse experimental models, from cell culture to patient-derived samples

Distributor Information

Non-Exclusive License available.