Monoclonal Antibodies Specific for Cardiac Muscarinic Acetylcholine Receptor

Biomaterial Description

This biomaterial consists of monoclonal antibodies directed against muscarinic acetylcholine receptors (mAChRs), a family of G-protein–coupled receptors that mediate parasympathetic neurotransmission throughout the body. Muscarinic receptors are widely expressed in the central nervous system and peripheral organs, where they regulate diverse physiological processes including cognition, smooth muscle contraction, glandular secretion, and cardiovascular function.

Among the five known subtypes (M1–M5), the M2 receptor is the predominant muscarinic receptor in the heart. It plays a critical role in controlling cardiac rhythm by coupling to inhibitory G-proteins (Gi), reducing cyclic AMP levels, and modulating ion channel activity. This receptor is essential for parasympathetic regulation of heart rate and contractility, making it a key target in studies of autonomic balance and arrhythmia.

The monoclonal antibodies described here were generated against purified porcine atrial muscarinic receptors and recognize distinct epitopes across the receptor protein. Collectively, these antibodies provide highly specific reagents for probing muscarinic receptor biology, advancing pharmacological research, and supporting translational studies in both neuroscience and cardiology.

Applications

-Receptor Identification & Subtype Mapping: Use antibodies to distinguish muscarinic receptor subtypes (M1–M5) in diverse tissues.

-Immunoprecipitation & Western Blotting: Isolate and detect muscarinic receptors from complex tissue or cell extracts.

-Immunohistochemistry & Imaging: Visualize receptor distribution and localization in cardiac tissue (M2 subtype) and other organs.

-Pharmacological Research: Assess drug-receptor interactions and receptor regulation mechanisms.

Advantages

-Broad Utility Across Assays: Antibodies can be applied in immunoprecipitation, immunoblotting, immunohistochemistry, and functional binding studies.

-Subtype Specificity: Recognize distinct epitopes on muscarinic receptors, enabling differentiation among receptor subtypes.

Distributor Information

Non-Exclusive License available.