PolyPhred

PolyPhred compares fluorescence-based sequences across traces obtained from different individuals to identify heterozygous sites for single nucleotide substitutions. PolyPhred functions are integrated with Phred, Phrap, and Consed-Autofinish, and is not a stand alone application. PolyPhred identifies potential heterozygotes using the base-calls, base-call qualities and peak sizes provided by Phred and the sequence alignments provided by Phrap. Potential heterozygotes identified by PolyPhred are tagged (color-coded) for rapid inspection using the Consed tool.

PolyPhred is available as a site license in an executable format (Linux, SGI-IRIX, Solaris, SunOS, HP-UX Mac-OSX, AIX and DEC-Alpha). PolyPhred operation requires Phred, Phrap and Consed-Autofinish software.

You will need to license Phred, Phrap, and Consed-Autofinish before obtaining PolyPhred.

For more information:  https://polyphred.gs.washington.edu/

International Requests:

University of Washington agreements require additional review if the requesting entity is located in, or affiliated with the government of, China (Including Hong Kong; not including Taiwan), Iran, North Korea, Russia, or Syria. For requests from these countries, please allow for an additional month of processing time for a response.

Available Licenses:

Academic License:

An academic license is available to users at academic and nonprofit research institutions at no charge. Please visit the developer's site to license the software.

Commercial License Procedure:

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